床旁肌钙蛋白I检测在检测临床上重要的犬心肌损伤方面具有可接受的性能，但在低浓度下精确度降低。 机翻

中文关键词（术语机标，非人工翻译）：实验室检查

摘要

To perform a partial validation of a point-of-care assay (Vcheck cardiac troponin I [VC-cTnI; cTnI]) for measurement of cTnI in canine serum and to perform a method comparison between the VC-cTnI and an established chemiluminescent assay (ADVIA Centaur XP High-Sensitivity Troponin I [AC-cTnI-HS]).

From September 4, 2024, through August 21, 2025, surplus canine serum was collected from a diagnostic laboratory, and within-day variability, dilutional parallelism, spiking recovery, and the effect of common interferents (hemolysis, icterus, and lipemia) were analyzed for the VC-cTnI assay. A method comparison of the VC-cTnI and AC-cTnI-HS assays was performed with 121 canine serum samples.

The within-day coefficient of variation ranged from 8.1% to 43.8%, decreasing as cTnI concentrations increased. Dilutional parallelism and spiking recovery assessments had mean ± SD observed-to-expected ratios of 80.5 ± 33.2% (range, 21.1% to 122.2%) and 112.8 ± 35.6% (range, 46.0% to 173.9%), respectively. There was no interference from icterus, hemolysis, or lipemia. There was a positive correlation between the VC-cTnI and AC-cTnI-HS assays (Spearman ρ, 0.742), though 16 of 121 samples (13.2%) were below the VC-cTnI detectable limit.

The VC-cTnI assay lacked precision for measurement of concentrations below 0.2 ng/mL, but within-day variability was acceptable for higher concentrations.

The VC-cTnI assay is more precise when measuring higher cTnI concentrations and can detect myocardial injury in dogs. Results in the normal and equivocal range of the manufacturer's reference interval may be more challenging to interpret because of suboptimal precision in this range.

摘要为 PubMed/NLM 公开许可元数据；全文版权归出版方所有，本平台不存储、不转载。